P-222: Expression of RFamide-Related Peptide-3 and KiSS-1 mRNA in The Hypothalamus of Pregnant Rat

Authors

  • Abli Moghadam AA
  • Jafarzadeh Shirazi MR
  • Kuhi Hoseinabadi O
  • Mohammadinejad S
  • Niazi A
  • Rahmanifar F
  • Sabet Sarvestani F
  • Tamadon A
  • Tanideh N
Abstract:

Background: There is no follicular development during pregnancy in the rat comparable to the changes of the estrous cycle. RFamide-related peptide-3 (RFRP-3) and kisspeptin were recognized as regulator of gonadotropin releasing hormone (GnRH) and luteinizing hormone (LH) secretion in several species, including the rat. The aim of the present study was to evaluate the relative expression of RFRP-3 and KiSS-1 mRNA in the hypothalamus of pregnant rats. Materials and Methods: Eighteen adult pregnant Sprague-Dawley rats (3-4 months old) were decapitated, and the whole hypothalamus was dissected at the age of 7, 14, and 21 days of pregnancy (n=6). The exact day of the rat pregnancy was confirmed using the vaginal smear method2. Four female non-pregnant rats were ovariectomized and assigned as control group. The dorsomedial hypothalamic nucleus (DMH) for detection of RFRP-3 mRNA and arcuate nucleus (ARC) for detection of KiSS-1 mRNA were separated from hypothalamus. Then relative expression of RFRP-3 and KiSS-1 mRNA were compared using real-time PCR method between control and pregnant groups. The data were subjected to the test of normality and analyzed by one-way ANOVA (SAS 9.1, SAS Institute Inc., NC), and mean separation was performed by Tukey&#039;s test at p=0.01. Results: The mean and SE of relative expression of RFRP-3 in DMH did not change during pregnancy (day 7, 40.8 ± 16.2%; day 14, 73.9 ± 17.7%; day 21, 49.9 ± 13.2%; p>0.01). However, the relative expression of KiSS-1 in ARC was the highest in day 7 of pregnancy and decreased up to day 21 of pregnancy (day 7, 17.1 ± 4.4%; day 14, 13.8 ± 5.3%; day 21, 4.3 ± 4.0%; p<0.01). Conclusion: Decrease of the GnRH and LH secretion during the pregnancy of rat may be controlled by constant expression of RFRP-3 mRNA and decreasing pattern of KiSS-1 mRNA expression.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

P-196: Lactation and mRNAs Expression of RFRP-3 and KiSS-1 in Hypothalamus of Rat

Background: Comparison of the effect of lactation on expression of RFRP-3 mRNA in dorsomedial hypothalamic nucleus (DMH) and KiSS-1 mRNA in arcuate nucleus (ARC) of hypothalamus in lactating and nonlactating rat Materials and Methods: Fourteen rats of the Sprague- Dawley strain were randomly allotted into three groups. The non-lactating rats (n=5) were separated from their pups immediately upon...

full text

Expression of RFamide-Related Peptide-3 (RFRP-3) mRNA in Dorsomedial Hypothalamic Nucleus and KiSS-1 mRNA in Arcuate Nucleus of Rat during Pregnancy

BACKGROUND RFamide-related peptide-3 (RFRP-3) and kisspeptin (KiSS-1) are known to respectively inhibit and stimulate gonadotropin releasing hormone (GnRH) and lute- inizing hormone (LH) secretion in rat. The aim of the present study was to evaluate the relative mRNA expression of RFRP-3 and KiSS-1 in the hypothalamus of pregnant rats. MATERIALS AND METHODS In a randomized controlled experime...

full text

Expression of RFamide-related peptide in the dorsomedial nucleus of hypothalamus during the estrous cycle of rats

Introduction: RFamide-related peptide (RFRP) is believed to act as an inhibitor of gonadotropin releasing hormone (GnRH) secretion. The aim of the present study was to compare the expression pattern of RFRP neurons in the dorsomedial nucleus of hypothalamus (DMH) at different phases of the rat estrous cycle. Methods: The phases of the estrous cycle were determined in 16 adult female Sprague-...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 7  issue 3

pages  123- 123

publication date 2013-09-01

By following a journal you will be notified via email when a new issue of this journal is published.

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023